MOTS-C

Description

MOTS-C (Mitochondrial-Derived Peptide) Overview

MOTS-c is a unique 16-amino acid peptide encoded by the mitochondrial genome rather than the nuclear genome. It acts as a signaling molecule that translocates to the nucleus in response to metabolic stress, where it regulates nuclear gene expression to promote metabolic homeostasis.

Research into MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA Type-c) centers on its role as a “mitokine.” It is extensively studied for its ability to mimic the physiological effects of exercise by enhancing fatty acid oxidation and improving glucose disposal. Unlike traditional metabolic agents, MOTS-c functions through a distinct mitochondrial-to-nuclear signaling pathway, making it a critical tool for longevity and metabolic research.

Technical Specifications
Peptide Name: MOTS-c

• • Amino Acid Sequence: Met-Arg-Trp-Gln-Glu-Met-Gly-Tyr-Ile-Phe-Tyr-Pro-Arg-Lys-Leu-Arg
  • CAS Number: 1627580-64-6
  • Molecular Formula: C101H152N28O22S2
  • Molar Mass: 2174.6 g/mol
  • Purity: ≥99.0% (determined by HPLC)
  • Appearance: White lyophilized powder
  • Solubility: Soluble in sterile water or bacteriostatic water

Mechanism of Action (Research Context)
MOTS-c is investigated for its multi-faceted approach to metabolic regulation:

• AMPK Activation: Research suggests MOTS-c increases levels of AICAR (an analog of AMP), which in turn activates the AMPK pathway. This is a central switch for energy metabolism and fat burning.
• Glucose Utilization: In experimental models, MOTS-c has been observed to promote GLUT4 translocation to the cell membrane, facilitating the uptake of glucose into skeletal muscle independently of the insulin pathway.
• Mitochondrial Biogenesis: Studies focus on MOTS-c’s ability to enhance mitochondrial function and density, potentially reversing age-associated metabolic decline.
• Folate Cycle Regulation: Unique to MOTS-c is its interaction with the folate cycle and de novo purine biosynthesis, which helps cells manage metabolic stress and maintain homeostasis.

Storage and Handling

• Lyophilized (Powder): Store at -20°C (-4°F) for up to 36 months. MOTS-c is remarkably stable in its freeze-dried state but should be kept away from light and moisture.
• Reconstituted (Liquid): Once reconstituted in a research solvent, the solution should be kept at 4°C (39°F) and utilized within 14 days. For longer storage of liquid MOTS-c, aliquoting and freezing at -20°C is recommended to avoid repeat freeze-thaw cycles.
• Handling: Standard laboratory safety protocols apply. This product is for laboratory research use only and not for human consumption.

Testing and Quality Control

At Flux Peptides, we ensure that your research is backed by chemical precision. Every batch of MOTS-c undergoes rigorous verification:

1. HPLC (High-Performance Liquid Chromatography): To confirm a purity profile of ≥99.0%.
2. MS (Mass Spectrometry): To verify the specific 16-amino acid sequence and molecular weight.
3. Solubility Testing: To ensure consistent reconstitution for experimental accuracy.